Upcoming events Metla

College Station, TX--Organized by:

• Nurul Islam-Faridi, PhD
  Research Molecular Cytogeneticist (USFS)
  Adjunct Professor (TAMU)
  


• Forest Tree Molecular Cytogenetics Laboratory


• Southern Institute of Forest Genetics
  Southern Research Station, U.S. Forest Service


• Department of Ecosystem Science & Management Texas A&M University

May 14 Monday   09:00 – 09:15 Welcome address by Dr. Tom Byram, Director, Western Gulf Forest Tree Improvement Program, Texas Forest Service & Texas A&M 09:15 – 10:00 FISH presentation (Emphasis on proper plant chromosome spreading) 10:10 – 11:00 Root process for enzymatic digestion 11:00 – 12:30 Root collection – Greenhouse 12:30 – 13:30 Lunch (on your own) 13:30 – 18:00 RNase Pre-treatment for FISH Fixation root tips Root processing – Enzymatic digestion – Slide preparation 18:00 – 19:30 Dinner on your own 19:30 – 22:00 Video Demo: Metafer (MetaSystems) – Automated detection of metaphase spreads & karyotyping     May 15 Tuesday   08:30 – 17:30 Practice on your own: Process roots Enzymatic digestion Slide preparation 9:00 – 10:00 FISH discussion – Methodology & Unforeseen problems 12:30 – 13:30 Lunch (on your own) 13:30 – 17:30 FISH Day – 1
Hybridization mixture preparation
Slide Denaturation (Chromosomal DNA)
Hybridization (DNA to DNA)
Overnight incubation
Set-up for hybridization site detection (FISH Day -2)     May 16 Wednesday   08:00 – 11:30 FISH Day – 2
Washing & Hybridization site(s) detection 11:30 – 12:30 Discussion: FISH with BAC, Transgene and genomic DNA probes; Pachytene FISH 12:30 – 13:30 Lunch (on your own) 13:30 – 18:00 Operation of epi-fluorescence microscope and use of MetaSystems FISH analysis software. We have two (State-of-the-Art)) epi-fluorescence microscopes (automated and manual) and will provide training for the both     May 17 Thursday   08:00 – 17:00 Nick-Translation – Cloned DNA
Check Labeled DNA on gel
View FISH slides, capture and process FISH images 12:30 – 13:30 Lunch (on your own) May 18 Friday   08:00 – 12:30 FISH slide analysis – Microscope
Metafer (MetaSystems Group Inc.) – automated detection of metaphase spreads 12:30 – 13:30 Lunch (on your own) 13:30 – 16:00 Capture & process FISH images 16:00 – 17:30 Question & Answer Session
Adjourn

PS:
Participants will be encouraged to submit a short report on the following headings within a week from May 18, 2012. Plant somatic chromosome preparation (root tip collection, pretreatment, fixation, enzymatic digestion and chromosome spread) and FISH (probe DNA, probe labeling and detection, RNase-A treatment, hybridization mixture, denaturation, hybridization and detection, capturing and pressing FISH image. The report should include two/three FISH images). For additional details please contact the workshop organizer.

E-mail: nfaridi@tamu.edu; telephone: 979-862-3908 (office), 979-845-2974 (lab).

News Release Science Contact:
News Release Science Contact:
News Release Science Contact: Claire Payne

From 2012-09-05 to 2012-09-07, Sheffield, United Kingdom, IUFRO Unit(s) involved: 9.03.00, 9.03.01
The Biodiversity and Landscape History Research Institute together with partners including the Ancient Tree Forum, International Oak Society, Tomasz Samojlik (Bialowieza Institute, Poland) and Mauro Agnoletti (IUFRO) are organising the conference to celebrate twenty years of work since our first major conference on the theme of ancient trees and woodlands. It is also almost ten years since our 2003 Working and Walking in the Footsteps of Ghosts event which started to raise issues and challenge assumptions about what is ‘ancient’ or ‘natural’ and what is meant by the terms ‘wood’ or ‘woodland’. Since then on-going work in a range of disciplines across ecology, biology, landscape history, archaeology, forestry and nature conservation has continued the process of research and evaluation across the subject area. The 2012 event will bring together key speakers in the debate and give the opportunity for others to present shorter papers or posters to explore themes and issues which will move the discussion forward and allow for a greater level of understanding between disciplines. We will include people from a variety of backgrounds (academic, NGOs, local authorities, conservation trusts, businesses and voluntary sector); and countries (principally UK and Europe).

From 2013-04-24 to 2013-04-26, Umeå, Sweden, IUFRO Unit(s) involved: 4.00.00
The conference is the final event of the FORSYS COST Action. FORSYS has brought together developers and users of forest Decision Support Systems (DSSs) from more than 30 countries. The 2013 international conference should gather expertise from different areas related to forest DSSs to form a comprehensive picture of problems and opportunities.

Introduction Peatlands are very common in all Nordic countries and they are known to accumulate more organic matter than other soils due to excess water and reduced decomposition rates. They also have particular microbial processes such as methane production that are not found in well-drained soil types, but are relevant for the global carbon balance; methane is an efficient greenhouse gas.

From 2013-04-18 to 2013-04-20, Toronto, On, Canada, IUFRO Unit(s) involved: 6.07.00
This three-day conference will cover a wide range of issues related to urban ecologies and political transgressions. It is designed for students, faculty, practitioners, community members, artists and researchers of all disciplines who are interested in enhancing their understanding of the various narratives that shape urban green spaces and the pedagogies that contribute to engaging with those diverse perspectives.

From 2013-10-07 to 2013-10-09, New Orleans, La, United States, IUFRO Unit(s) involved: 4.03.00
A forest is the most complex of the terrestrial ecosystems, and significant efforts have been made to understand and model the mechanisms undelying its behavior. The technological advancements of the last two decades have allowed development of sophisticated approaches to assessing complexity as well as integrating scale, measurements and accuracy into the understanding of response of the forest to different stimuli. The purpose of this conference is to introduce the latest advances in representation, quantification, measurement, analysis, and modeling of forest cover “from tree to landscape”.

From 2013-05-16 to 2013-05-21, Estoril, Portugal And Other Locations, IUFRO Unit(s) involved: 8.00.00, 4.00.00
It is the aim of the conference to investigate the contribution of planted forests to sustainable development in the context of global changes. Central topics would include the sustainability of planted forests in the context of changing climates and the future role of planted forests in envi ronmental protection and REDD+. The aim is to address issues surrounding planted forests today, their productive, environmental and social functions and the challenges they present. The target audience includes forest scientists, policy makers, the private forestry sector and forest managers.

From 2012-05-10 to 2012-05-13, Sliac, Slovakia, IUFRO Unit(s) involved: 4.03.03
The Conference is a joint event organized by the Technical University in Zvolen and COST FP 804 FORSYS. It is a part of the solution of projects “Precision Forestry Principles and Methods Research (TUZVO)” and “Centre of Excellence for Decision Support in Forest and Landscape (TUZVO)". There is the FORSYS Spring 2012 MC/WG meeting organized simultaneously. The conference is held on 10–13 May 2012 in Sliac, the Slovak Republic. The aim of the conference is to present new ideas and tools supporting the decision making process of forest managers and forestry researchers.